| Name | Pos | Birth | Nat |
|---|---|---|---|
| Lars Unnerstall | GK | 20/07/90 | Germany |
| Remko Pasveer | GK | 08/11/83 | Netherlands |
| Issam El Maach | GK | 01/02/00 | Morocco |
| Yannick Gerritsen | GK | 14/02/06 | Netherlands |
| Robin Pröpper | DF | 23/09/93 | Netherlands |
| Bart van Rooij | DF | 26/05/01 | Netherlands |
| Mees Hilgers | DF | 13/05/01 | Indonesia |
| Max Bruns | DF | 06/11/02 | Netherlands |
| Aske Adelgaard | DF | 10/11/03 | Denmark |
| Stav Lemkin | DF | 02/04/03 | Israel |
| Juliën Mesbahi | DF | 31/01/06 | Morocco |
| Krzysztof Kurowski | DF | 29/08/06 | Poland |
| Guilherme Peixoto | DF | 25/05/06 | Portugal |
| Ruud Nijstad | DF | 26/05/08 | Netherlands |
| Arno Verschueren | MF | 08/04/97 | Belgium |
| Thomas van den Belt | MF | 18/06/01 | Netherlands |
| Ramiz Zerrouki | MF | 26/05/98 | Algeria |
| Mathias Kjølø | MF | 27/06/01 | Norway |
| Kristian Hlynsson | MF | 23/01/04 | Iceland |
| Gijs Besselink | MF | 16/06/04 | Netherlands |
| Daouda Weidmann | MF | 04/05/03 | France |
| Harrie Kuster | MF | 11/09/05 | Netherlands |
| Marko Pjaca | FW | 06/05/95 | Croatia |
| Sam Lammers | FW | 30/04/97 | Netherlands |
| Wout Weghorst | FW | 07/08/92 | Netherlands |
| Naci Ünüvar | FW | 13/06/03 | Turkey |
| Taylor Booth | FW | 31/05/01 | USA |
| Sayfallah Ltaief | FW | 22/04/00 | Tunisia |
| Daan Rots | FW | 25/07/01 | Netherlands |
| Younes Taha | FW | 27/11/02 | Morocco |
| Lucas Vennegoor of Hesselink | FW | 14/03/06 | Netherlands |
| Sondre Ørjasæter | FW | 28/11/03 | Norway |
FC Twente '65
Stadium: De Grolsch Veste
Foundation: 1965